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Decreasing cell division


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#1 Lfs

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Posted 12 February 2014 - 10:49 AM

Hello!

I would like to know if there is a way to decrease cell proliferation without decrease cell viability (take out some nutrient)...

I'm working with HEK293 and I need to transfect these cells and maintain them for 4 to 7 days with a high (or not so high) protein expression. Unfortunately, I don't have time to establish stable cell lines. Maybe changing serum %?


Thanks in advance!

#2 bob1

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Posted 12 February 2014 - 11:25 AM

Anything that slows proliferation is also likely to have an effect on viability, however, the most common way, and probably the least damaging to most cells is to arrest them at G0 by serum starvation.  24-48 hours in 0% FBS works for most cell lines.






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