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Mitomycin C Treated Feeder Cell

culture media tissue culture cell culture feeder cell keratinocytes

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#1 roxx_gurl

roxx_gurl

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Posted 11 February 2014 - 09:56 PM

Hi all, 

 

 

My supervisor requested me to have some feeder cell to support my primary oral keratinocytes growth (which has been failed despite using both explant and enzymatic technique). However, I found few protocol from website and they treated cell with mitomycin C. I have no idea which product of mitomycin C should I used. I tried Sigma but the product has been discontinued. It might be helpful if anyone has treated cell with mitomycin C and produced feeder cell. 

 

However I need to optimize the protocol as well, since I am planning to treat my primary oral fibroblast with mitomycin C. Should I just purchase the feeder cell from supplier or it is not worth it to do so

 

Thanks







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