My supervisor requested me to have some feeder cell to support my primary oral keratinocytes growth (which has been failed despite using both explant and enzymatic technique). However, I found few protocol from website and they treated cell with mitomycin C. I have no idea which product of mitomycin C should I used. I tried Sigma but the product has been discontinued. It might be helpful if anyone has treated cell with mitomycin C and produced feeder cell.
However I need to optimize the protocol as well, since I am planning to treat my primary oral fibroblast with mitomycin C. Should I just purchase the feeder cell from supplier or it is not worth it to do so