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Lentiviral transduction of human epithelial cells

Lentiviral transduction MOI Stable clone Transgene integration Titer

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#1 Epigeneticist

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Posted 11 February 2014 - 07:36 PM

Besides possibly killing your cells, if you infect with lentivirus at a high MOI, will your gene of interest be integrated more than once (i.e. cells with high transgene copy number)? Is it a fine balance between making sure all cells are infected but also that not too much virus is being introduced that cells will have high copy number?

 

I plan to produce lentivirus from transfecting HEK293T cells. What is the best way to titer this virus? ELISA, qPCR or some other method?

 

Thanks!







Also tagged with one or more of these keywords: Lentiviral transduction, MOI, Stable clone, Transgene integration, Titer

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