Besides possibly killing your cells, if you infect with lentivirus at a high MOI, will your gene of interest be integrated more than once (i.e. cells with high transgene copy number)? Is it a fine balance between making sure all cells are infected but also that not too much virus is being introduced that cells will have high copy number?
I plan to produce lentivirus from transfecting HEK293T cells. What is the best way to titer this virus? ELISA, qPCR or some other method?