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Lentiviral transduction of human epithelial cells

Lentiviral transduction MOI Stable clone Transgene integration Titer

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#1 5280

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Posted 11 February 2014 - 07:36 PM

Besides possibly killing your cells, if you infect with lentivirus at a high MOI, will your gene of interest be integrated more than once (i.e. cells with high transgene copy number)? Is it a fine balance between making sure all cells are infected but also that not too much virus is being introduced that cells will have high copy number?

 

I plan to produce lentivirus from transfecting HEK293T cells. What is the best way to titer this virus? ELISA, qPCR or some other method?

 

Thanks!







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