I have been trying to image cells stained with phalloidin tagged with Alexa 647 using TIRF, but the signal is very low compared to cells stained with phalloidin with Alexa 488. I use the exact same protocol to prepare the cells in both cases, but for some reason, the Alexa 647 comes out very dim.
I have attached images for each below.
Any one have any ideas as to why this is?
Edited by alphawhiskey, 11 February 2014 - 09:17 AM.