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How efficient is the 2A sequence


Best Answer Ahrenhase, 16 February 2014 - 08:53 AM

Arg.. I'm not in the lab at the moment So I cannot show you the papers I found on the subject..

 

But if memory serves T2A gives you cleavage something like 90%-95% of the time. While P2A gives you something like 95%-100%. (At least in the cell lines that were used in the papers I looked at.)

 

Those two 2A peptides are very good (at least in the cells lines the studies were conducted in).

 

The other types of 2A peptides are less efficient..

Good deal, thanks

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#1 Ahrenhase

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Posted 10 February 2014 - 12:23 PM

If I have 3 ORFs separated by two 2A sequences, will I get 3 single proteins 100% of the time?



#2 perneseblue

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Posted 12 February 2014 - 06:42 PM

Arg.. I'm not in the lab at the moment So I cannot show you the papers I found on the subject..

 

But if memory serves T2A gives you cleavage something like 90%-95% of the time. While P2A gives you something like 95%-100%. (At least in the cell lines that were used in the papers I looked at.)

 

Those two 2A peptides are very good (at least in the cells lines the studies were conducted in).

 

The other types of 2A peptides are less efficient..


May your PCR products be long, your protocols short and your boss on holiday

#3 Ahrenhase

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Posted 16 February 2014 - 08:53 AM   Best Answer

Arg.. I'm not in the lab at the moment So I cannot show you the papers I found on the subject..

 

But if memory serves T2A gives you cleavage something like 90%-95% of the time. While P2A gives you something like 95%-100%. (At least in the cell lines that were used in the papers I looked at.)

 

Those two 2A peptides are very good (at least in the cells lines the studies were conducted in).

 

The other types of 2A peptides are less efficient..

Good deal, thanks






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