Which gene is the best internal control for RT-PCR
Posted 25 May 2004 - 01:58 PM
I want to know which gene is the best internal control for RT-PCR. I usually use b-actin as a control, however, it sometimes seems to express differently in cells after certain treatment. I am not sure if the changes are really due to regulated expression or just technical issues.
Posted 25 May 2004 - 04:19 PM
5S rRNA is the best control we use to correct this problem.
Posted 26 May 2004 - 01:39 PM
Edited by pcrman, 26 May 2004 - 09:42 PM.
Posted 07 June 2004 - 07:42 PM