Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Glycogen affect restriction enzyme digestion?


  • Please log in to reply
1 reply to this topic

#1 biofingers

biofingers

    member

  • Active Members
  • Pip
  • 12 posts
1
Neutral

Posted 08 February 2014 - 06:04 AM

Hi, I am wondering if too much glycogen (say 1ug/ul) will have effect on restriction enzyme digestion or not? I think the enzyme should work but I am not sure will the glycogen create star activity? 

 

 



#2 perneseblue

perneseblue

    Unlimited ligation works!

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 585 posts
20
Excellent

Posted 08 February 2014 - 11:53 AM

I know common RE (Xba, BamHI, EcoRI, XhoI) do work in the presence glycogen. However I no experience with the more exotic RE, the ones that need to cut as a dimer.

 

I have also never experienced Star activity myself before, like due to the fact I tend to cut my DNA in volumes no smaller than 20ul with no more 5% enzymes total.

 

So I would recommend the digest be cut in a bigger volume if you are concern about star activity.


May your PCR products be long, your protocols short and your boss on holiday




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.