Posted 26 April 2014 - 12:11 PM
Not to forget a different buffer...e.g. Lithium borate, then you can increase voltage (150 V for a 2.5% gel should work) without heating up. This reduces running time (more convenient and less diffusion) and you get sharper bands.
And it's cheaper...
just curious: if it is cheaper and better, than why do people keep using other buffers than the lithium borate?
I guess mostly out of tradition or habit, they learnt gel electrophoresis with TAE or TBE, have the buffers and recipes and protocols and don't need to think about with standard work. A few drawbacks are there with such borate buffers too: high molecular DNA is not resolved as well, some dyes are not working. Perhaps also issues with a few downstream applications, which I don't know (DNA extraction and purification out of the gel not).
And finally it's a not that well known and common buffer.
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.