I have two suspension cell lines of small cell lung carcinoma in culture. When I got them in my laboratory they was very sensitive to poly-L-lysine and the day after the seeding in a treated flask, they appeared adherent, with the typical poligonal aspect of adherent cells. Then we had problem with liquid nitrogen in our laboratory and we lost all frozen stock, therefore I made a stock from the cultured cells. Now I have necessity of work with adherent cells, but when I thaw them and seed in treated flasks, they not appear to adhere completely, although they replicate and respond positively to MTT assay. They appear round rather than poligonal.
Could be, their insensitive to poly-L-lysine, due to a sort of injury of my cell line? what do you suggest me: to buy new cell lines or to try an alternative to poly-L-lysine??
Thank you in advance for your suggestion.