well, as no one had an answer till the moment ... here what was on our minds ...
" You will see that the TAMRA readings were as follows :-
1- NTC : no Ct
2- stock : no Ct
3- dilution 1:10 No Ct
4- dilution 1:100 Ct 16.17
5- dilution 1:1000 Ct 20.04
6- dilution 1:10000 no CT "
we can explain these readings that the stock and dilution 1:10 were too concentrated to be detected in contrast to dilution 1:10000 that was too diluted ...
if am wrong please lead me ...
if that's true ; then our problem is hardware thing; am worried i missed something while setting the run..
what the company suggested ; will drive us to go and buy PCR plates instead of using the PCR tubes we already have ...
which means more money ...
another thing :-
anyone worked with these plates ?? i have to seal them for sure ... and i think i can split them so i wouldn't need to use a whole new plate each time ...
but going to what the company suggested i need to have a whole plate always there to decrease the light reflection from the gold block ..
so, i will use the same plate for many times ... and what remains in the tubes after the run .. what shall i do with them ???
pipett them out ???
anyone experienced such thing ???
will be in extreme pleasure if anyone can lend a hand ..
p.s : am setting a new reaction these days hope to see something !