Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

How to view the encapsulated cells under microscope?

microencapsulation bacteria microscope microbiology

  • Please log in to reply
5 replies to this topic

#1 Celz

Celz

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 78 posts
3
Neutral

Posted 05 February 2014 - 01:17 AM

Dear all, 

 

Did anyone have any idea how can I view the encapsulated cells under light microscope? How can I make the protective layer become more visible so that I can compare the differences between non-encapsulated and encapsulated cells? Thank you 



#2 pcrman

pcrman

    Epigenetist

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,165 posts
68
Excellent

Posted 05 February 2014 - 01:19 AM

What are encapsulated cells? 



#3 Celz

Celz

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 78 posts
3
Neutral

Posted 05 February 2014 - 01:38 AM

The bacterial cells that being encapsulated by a protective layer, such as maltodextrin and gelatin. 



#4 Phil Geis

Phil Geis

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 218 posts
15
Good

Posted 05 February 2014 - 09:23 AM

Use India ink prep to see the capsule.  

http://web2.uwindsor...psule_Stain.htm

 

You can typically remove the capsule with a dilute solution of NaEDTA.



#5 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,798 posts
406
Excellent

Posted 05 February 2014 - 01:04 PM

The classic stain for capsules is rose-bengal followed by nigrosin, the bugs will stain bright pink and the capsule will appear as a clear ring around the bacteria against the dark background.



#6 Celz

Celz

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 78 posts
3
Neutral

Posted 06 February 2014 - 05:16 PM

Thanks for the protocol. I was using Anthony's staining method to view the encapsulated bacterial cells. I have a question that would like to seek for your advice. Attached is the cells image after staining with crystal violet

 

First image (IMG_6985) is the bacterial cells incubated with maltodextrin and fructo-oligosaccharide. Did anyone know what is that pinkish stuff? Is maltodextrin or fructo-oligosaccharide? Or the combination of both of them?

 

Second image (IMG_7007) is the bacterial cells incubated with maltodextrin only. I noticed there is a number of sphere shape capsule-like compound. Is that the encapsulated bacterial cells? 

 

Thank you. 

Attached Thumbnails

  • IMG_6985.JPG
  • IMG_7007.JPG






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.