Thanks for the protocol. I was using Anthony's staining method to view the encapsulated bacterial cells. I have a question that would like to seek for your advice. Attached is the cells image after staining with crystal violet.
First image (IMG_6985) is the bacterial cells incubated with maltodextrin and fructo-oligosaccharide. Did anyone know what is that pinkish stuff? Is maltodextrin or fructo-oligosaccharide? Or the combination of both of them?
Second image (IMG_7007) is the bacterial cells incubated with maltodextrin only. I noticed there is a number of sphere shape capsule-like compound. Is that the encapsulated bacterial cells?