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Totipotent Stem Cell From Molecular Components


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#1 caters

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Posted 01 February 2014 - 10:18 PM

Q1 I am wanting to make an animal cell from DNA, RNA, Protein, and Fatty Acids as well as Glucose and other sugars. I am wondering. Should I start with the cell membrane or the nucleus and nucleolus(both are very important)?

 

Q2 The pace of DNA replication is 8 2/3 hours for every chromosome and 2097152 of each chromosome in 1 week. Is that just an estimate because I know that it is 8 2/3 hours per replication origin.

 

Q3 Once I know how many proteins of a particular type should I do this:

 

Go to BioGPS

 

Look up the proteins

 

Make sure I have human selected(since I am making a human stem cell)

 

Average the mRNA expression to get stem cell mRNA expression

 

Multiply by 100 to get the amount of protein

 

Q4 I have heard of amplicons used in PCR. Do I really need one to copy a whole gene or just primers, DNA, and enzymes?

 

 



#2 doxorubicin

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Posted 02 February 2014 - 12:59 AM

So you are trying to be the first person to create life from a collection of components, and you are asking for advice here.  Sounds like an exam question to me!



#3 caters

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Posted 02 February 2014 - 09:45 AM

Yes I am trying to be the first person to make life from its components. Those 4 questions I have are relevant to this and not all are like an exam question(such as the one related to gene expression by strands of mRNA which is the easiest way)


Edited by caters, 02 February 2014 - 02:38 PM.





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