Does anyone here work with gateway technology?
If so: would it be best to introduce the gatewaysequences after creating the ideal vector or the other way around and make it gatewaycompatible first and then introduce the C and or N tags?
The latter seems harder because I have a gateway vector however, I dont have restriction sites to cut the vector anymore after the gateway sequences. Or is there another way to cut the vector?
Edited by lucilius, 01 February 2014 - 07:21 AM.