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Why cells are floating after incubation in EBSS??

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#1 lilin_hust



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Posted 31 January 2014 - 06:03 PM

Has anyone every use EBSS to starve cell to induce autophagy? I use HEK293 cell, do transfection, and then treat with EBSS to starve cell. The cells are floating even treat with EBSS just for 1 hr.


What's the problem? Has anyone of you have any idea about this? Thanks you all so much!!!



#2 bob1


    Thelymitra pulchella

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Posted 31 January 2014 - 11:52 PM

293 are notorious for being weakly attached, the floating may be a result of the treatment. You could try coating the plates with collagin or gelatin.

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