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maxi culture

Bacterial culture

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#1 M.B.T



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Posted 31 January 2014 - 11:27 AM

Hi everyone,

Edited by Neda, 31 January 2014 - 04:16 PM.

#2 bob1


    Thelymitra pulchella

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Posted 31 January 2014 - 01:27 PM

For liquid cultures like this is, it is recommended to do a short liquid culture that you would then use to inoculate the larger liquid culture, which you will do the maxiprep from. The reason is due to growth curves - picking a colony and then adding it to a large volume of medium results in a very long lag phase, but inoculating with already growing bugs results in a shorter lag phase and with the right incubation times and temperatures, gives you maximal plasmid yield.

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