I infected two cells with IAV using two different MOI, one of them 1 and the other is 10. Now I wanted to compare the expression level of one miRNA in both cells. I think this is not right to compare. Since I did the experiment, has anyone any suggestion to surmount or overcome this mistake without repeating the experiment. any suggestion
Different MOI in comparison experimentViro
Posted 31 January 2014 - 01:29 PM
Why is it not right - as far as I can see, all you are comparing is the difference between two different infection amounts, so there shouldn't be a problem. The results can be normalized to a cellular gene that isn't affected by the infection.
Posted 31 January 2014 - 06:51 PM
But the problem I think is that when u hit the cells with a high MOI, more cells died while still a live in the other low MOI and this affect the expression of cell genes. Which to normalize to a gene that not affected by infection ? like B actin For example ?
Posted 31 January 2014 - 11:50 PM
Yes, dying cellare a problem, however DNA and RNA are rapidly degraded during apoptosis.
As to the gene - check the literature. many viruses alter actin expression.