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Is PI fixable in flow citometry?

PI Flow cytometry PFA

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#1 BioPe

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Posted 30 January 2014 - 10:58 AM

hey,

 

After staining cells with propidium iodide (viablility), can we fix the cells with PFA for analysis later on?

 

Thanks,

BioPe.



#2 Tabaluga

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Posted 30 January 2014 - 11:14 AM

I learned the time between PI addition and measurement should be relatively short, so I doubt storing the samples by fixing would be a good idea...in this thread here there is someone who apparently tried, though: https://lists.purdue...ust/037730.html

Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.

 


#3 bob1

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Posted 30 January 2014 - 12:02 PM

The PI will be washed out with more and more washes/buffer changes, but it is certainly possible to PI stain, collect the samples again (if you have the right sort of FACS machine) and then subsequently fix and probe.

 

Note- I don't do FACS so not sure if this would be true - if you did PI staining and then fixed the cells, you should be able to still do live/dead immediately after fixing, as only the dead cells prior to fixing should have taken up the PI.







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