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program to match sequences


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#1 lyok

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Posted 29 January 2014 - 11:27 AM

Hallo all,

 

I am looking for a program that I can use to check whether certain sequences are present in my plamids.

 

The problem is: when 1 amino acid is different than I can not find the sequence in my plasmid.

 

I want a program that, for example, would still "match" the sequences while mentioning that 1 base is different.

 

for example, the sequence of my plasmid is:

 

... TGTGTCCCTAAATCGT....

 

and the "gene" is: TGTGTCCGT, than I would not find it because I have that G rather than a C.

 

Any ideas or suggestions?

I just want to check for certain genes even if there is a small difference.

 

 

 

 



#2 hobglobin

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Posted 29 January 2014 - 12:31 PM

perhaps with BLAST and the megablast option?

http://blast.ncbi.nl...K_LOC=blasthome


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#3 mboss

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Posted 29 January 2014 - 12:39 PM

You can use clustalw. If you are a windows user it is included in BioEdit which is free. There afe also many other options, you're basically looking for a sequence alignment program.



#4 lyok

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Posted 29 January 2014 - 01:17 PM

You can use clustalw. If you are a windows user it is included in BioEdit which is free. There afe also many other options, you're basically looking for a sequence alignment program.

Ok, I'll check that one.

 

 

perhaps with BLAST and the megablast option?

http://blast.ncbi.nl...K_LOC=blasthome

No, because I want to compare my sequence with sequences from certain genes.

Or is there a way I can do this using NCBI?


 

I have 1 sequence (the plasmid) and I want to find certain genes in this plasmid (like for example a drug resistance gene).

I want to match those genes woth the plasmid (see if the gene is really in the plasmid or not)






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