Dear Thelymitra pulchella，
May I know is there any way to recover back the bacteria to make it become active? Thanks
You can select for fast growing strains by just doing growth curves on lots of different isolates, and repeatedly choosing only those that grow faster than others. For incorrect storage - you should get the bacteria growing actively again and then make more stocks under appropriate conditions for your bacteria.
Do you think the bacteria can be activate back if I culture it onto the agar plate and isolate the single colony? Thank you.
Its not so much about "activating" them back, its more about selecting the right isolate again...
Some will be fast growers, others slow growers, its just up to you to get that fast growing one back...
(and yes, some might indeed be slow growers, but due to a mutation become fast growers..)