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Centrifuge stopped working while my blood samples were incubated in Cell lysis s

DNA Extraction Urgent Promega

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#1 hercolanium

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Posted 27 January 2014 - 02:30 AM

Hi, my centrifuge stopped working before I could spin my samples that are incubated in Cell Lysis solution, I am using Promega DNA purification kit and I don't have access to another centrifuge until tomorrw, will my samples be lost or can I continue with the procedure tomorrow?

Please help and thanks in advance


Edited by hercolanium, 27 January 2014 - 05:43 AM.


#2 DeeAnnVisk

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Posted 27 January 2014 - 08:57 AM

Oh Dear!  So sorry to hear about this.  What is the endpoint of your experiment?  Isolating RNA or protein?  Probably lost by now

 

If you are after genomic DNA then you may be able to rescue.  Try looking at the manufacturer's website and/or contact them for assistace.

 

Troubleshooting centrifuge:  turn all the way off (unplug if you have to!)  Let it rest at least 10 seconds, then turn it on again.  May also try finding the manual for the centrifuge--either hard copy in the lab or online.

 

Good luck!







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