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Chemiluminescence vs. Fluorescence visualisation of western blot


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#1 science noob

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Posted 26 January 2014 - 04:54 PM

Has anyone used a fluorescently-tagged secondary antibody instead of chemiluminescent secondaries to visualise bands on a western blot membrane? 

 

Would it be more sensitive and hence more accurate compared to the conventional method of visualising bands?



#2 bob1

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Posted 26 January 2014 - 08:15 PM

Yes, and Yes...

 

The usual fluorescent antibodies used are infra-red range antibodies, and are purported to have a greater sensitivity and a more linear response over a wider range of antibody binding (i.e. no chemi reaction to take into account).






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