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Chemiluminescence vs. Fluorescence visualisation of western blot

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#1 science noob

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Posted 26 January 2014 - 04:54 PM

Has anyone used a fluorescently-tagged secondary antibody instead of chemiluminescent secondaries to visualise bands on a western blot membrane? 


Would it be more sensitive and hence more accurate compared to the conventional method of visualising bands?

#2 bob1


    Thelymitra pulchella

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Posted 26 January 2014 - 08:15 PM

Yes, and Yes...


The usual fluorescent antibodies used are infra-red range antibodies, and are purported to have a greater sensitivity and a more linear response over a wider range of antibody binding (i.e. no chemi reaction to take into account).

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