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#1 Refef



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Posted 26 January 2014 - 08:53 AM

Does anyone has an idea about affecting low temperature against phenol in DNA extraction?

Normally, I have used phenol:chloroform:isoamyl alcohol with 4c centrifuge according to my protocol? But I wonder, what is the advantage of 4c? because I know phenol in low temperate evaporated. 

#2 jerryshelly1



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Posted 31 January 2014 - 10:25 AM

I can only answer this with... Phenol can oxidize at it ages (this is a slow process) and keeping the solution at 4C increases its lifetime. I would also say that storing the above solution at 4C keeps the isoamyl alcohol in solution which is necessary to prevent excessive foaming of your phenol/chloroform during mixing.


Edit: One more thing... Going from 4C to a RT centrifuge may cause the cap to open during spinning.

#3 hobglobin


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Posted 31 January 2014 - 10:29 AM

I once used a protocol where it was centrifuged in a "normal" centrifuge at room temperature and it worked too. Therefore yours might be an overcautious protocol.

One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.

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