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In 2^ minus ddCt method, why is the "minus" sign?

qPCR Data Analysis Relative Quantitation 2^ddCt Method

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#1 Serratia



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Posted 24 January 2014 - 06:12 AM

For the qPCR relative quantitation, we use the 2^-ddCt method. I was wondering why the minus sign before ddCt?

#2 Tabaluga


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Posted 24 January 2014 - 12:32 PM

If I understood it correctly, ddCt is dCt(sample) - dCt(reference), -ddCt would be -(dCt(sample) - dCt(reference)), which equals dCt(reference) - dCt(sample), so it's just another way of calculating.

But I have also seen ddCt referred to as dCt(reference) - dCt(sample), then it would be the other way round...

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