I am going to add 6histag in C-terminal of my target protein. It is my first time to insert such big pieces in a gene. I wonder which protocol could be useful for this purpose? I have used QuikChange II XL Site-Directed Mutagenesis before to add 6 nts. Will it work in this case?
In addition, I was told several aminoacids should be added between his-tag and target protein. Normally how many and which kinds of aminoacids should I add into? Are there other points I have to keep in mind for such insertion? Many thanks in advance!