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Cleaning of cuvettes (spectrophotometer) for RNA measurement


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7 replies to this topic

#1 Kongen

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Posted 23 January 2014 - 10:01 AM

Hi People.

 

I have always just been measuring concentrations on a nanodrop so never really thought about doing it on the spec. However at my current location I have to measure this in a spec. So my question is for you who have experience in this, how much cleaning between samples are needed? There is only two cuvettes accesable for RNA/DNA measurements. And what solution do you use for cleaning? EtOH? NaOH,Tris-, EDTA combi?



#2 phage434

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Posted 23 January 2014 - 02:09 PM

Are you attempting to recover samples after the reading or not?



#3 mdfenko

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Posted 24 January 2014 - 04:29 AM

are you using plastic or glass or quartz cuvettes?

 

do you have a cuvette cleaner (connected to vacuum, with a side-arm flask)?

 

how (and how often) do you clean the nanodrop head?

 

(btw- just because i'm a nitpicker- the nanodrop is a spectrophotometer, just doesn't use cuvettes).


talent does what it can
genius does what it must
i do what i get paid to do

#4 Kongen

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Posted 24 January 2014 - 05:12 AM

No, im not trying to recover the samples. I use quartz, dont have a cuvette cleaner. And true that, and must admit I have never cleaned it, I wasnt responsible for maintaining it, so i have basically just been wiping it between uses with water.



#5 mdfenko

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Posted 24 January 2014 - 12:18 PM

you may want to purchase a cuvette cleaner (here's one place).

 

we flush with water then dry by flushing with ethanol (95%) or acetone and allowing to dry on the cleaner.


talent does what it can
genius does what it must
i do what i get paid to do

#6 rkay447

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Posted 24 January 2014 - 01:27 PM

I just rinse a few times with sterile water (fill, give a good flick of the wrist to empty).  I was taught never use EtOH on the quartz since it can precipitate DNA onto the glass.  



#7 mdfenko

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Posted 25 January 2014 - 02:39 PM

after flushing with water there should be no dna to precipitate. the ethanol (or acetone) are to speed drying.


talent does what it can
genius does what it must
i do what i get paid to do

#8 memari

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Posted 26 January 2014 - 09:53 AM

all above are obsession.

once rinsing with water is enough. 


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Babak Memari




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