I wonder which is the best way to store my ligation reaction over the weekend.
I will do an overnight ligation at 15°C and I need the ligated vector on monday morning for electroporation of e. coli.
I tend to keep the ligation reaction at 4°C from friday until monday, then purify the DNA with a PCR purification kit, elute it in water and use it for the electroporation.
Would you rather freeze the ligation reaction on friday? Or do the DNA purification on friday and freeze it eluted in water?
I think freezing and thawing the DNA does damage it more than storing it at 4°C in the ligation reaction.
What do you think?