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Crosslinking on frozen samples (brain tissue)

ChIP crosslink

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#1 mosso2000



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Posted 23 January 2014 - 01:33 AM

Hi everyone, I was wondering if it is actually reasonnable to flash freeze samples (in my cain, brain tissues) in liquid nitrogen, store them at -80C for a few weeks and then crosslink them after thawing? I thought this wasn't a good idea but I've seen few papers describing such procedure... Thanks for sharing your opinion/experience!

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