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flow cyto, problem with dot plot on cell quest - urgent help needed

flow cytometry cell quest dot plot colours FACScan

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#1 AllyPhD



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Posted 22 January 2014 - 05:44 AM



I'm a PhD student who is desperatly stuck with a problem with my flow cytometry work.


I have run a sample of monocytes through the analyser and produced a dot plot, histogram, density plot and stats plot which has worked well using CellQuest Pro. However when I try and print my results the events (blobs) from the dot plot are missing at it appears like a clear template. (Yet they other graphs work fine?)


Having done some fiddling about i have noticed that regardles of what colour i select the events to show up as on the dot plot they remain white; where as the denisty plot and other graphs change colour to the one selected instantly. 


I have made sure the colour pallet is working and tried everything i can think of to get it to work but I'm having no luck.


I'm sure there must be a simple solution - can anyone help?


Thank  you very much! 


Ally (Chester) sad.png

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