Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

flow cyto, problem with dot plot on cell quest - urgent help needed

flow cytometry cell quest dot plot colours FACScan

  • Please log in to reply
No replies to this topic

#1 AllyPhD

AllyPhD

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 22 January 2014 - 05:44 AM

Hi,

 

I'm a PhD student who is desperatly stuck with a problem with my flow cytometry work.

 

I have run a sample of monocytes through the analyser and produced a dot plot, histogram, density plot and stats plot which has worked well using CellQuest Pro. However when I try and print my results the events (blobs) from the dot plot are missing at it appears like a clear template. (Yet they other graphs work fine?)

 

Having done some fiddling about i have noticed that regardles of what colour i select the events to show up as on the dot plot they remain white; where as the denisty plot and other graphs change colour to the one selected instantly. 

 

I have made sure the colour pallet is working and tried everything i can think of to get it to work but I'm having no luck.

 

I'm sure there must be a simple solution - can anyone help?

 

Thank  you very much! 

 

Ally (Chester) sad.png







Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.