Posted 21 January 2014 - 02:17 AM
Posted 21 January 2014 - 08:41 AM
If you are only concerned about some cytokine/protein that is being excreted into your media then you should just remove the liquid without disturbing they adherent cells. Depending on what you are looking for, you may want to filter your cells to remove any of the larger particles (dead cells) that could alter your overall protein concentration. From here, you could spin down your cells and isolate your protein.
Can you be a little clearer in what you are looking for?