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Reading Oligonucleotides Information

primer tm

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#1 Lelamekala Vengidasan

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Posted 21 January 2014 - 12:32 AM

Good evening. I need a help to read the information on the primer information sheet. 

What is the different between Tm(1M Na+) and Tm(50mM Na+)? 

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Edited by Lelamekala Vengidasan, 21 January 2014 - 12:33 AM.


#2 mdfenko

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Posted 21 January 2014 - 04:51 AM

a factor influencing melting temperature is ionic strength of the solution (and the specific ion).


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