I had set a restriction digestion of pUC19 with Kpn1 and the plasmid was linearized. I wanted to cut the plasmid with EcoR1 and therefore i precipitated the DNA with 3M Sodium acetate pH4.5 and ethanol. I checked the plasmid DNA concentration on gel, loss of DNA was very evident. I set the EcoR1 digestion with the precipitated DNA and now only a faint band can be seen.
1. Is 3M Sodium acetate ethanol a good method for plasmid DNA precipitation? Is there any good method that gives a good DNA yeild (Ive heard of ammonium acetate but dont have the protocol for it)
2. Which is a good method for extraction of DNA from agarose gels (Phenol chloroform treatment ?
i dont have spin columns/ gel extraction columns for plasmid/PCR purification