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Glutamine synthtase amplification

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#1 lalehr



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Posted 20 January 2014 - 02:13 AM

Hi everyone


I am going to start glutamine synthetase gene amplification to overexpress a protein in CHO-K1 cells. I have noticed that some papers have used the GS minigene and the others employed GS cDNA. I was wonedring which sequence is more efficient for amplification and i also cant find the exact sequence for the GS minigene. although the minigene has been used in pEE14 expression vectors but I dont have access to the sequence. I reaaly appreciate any suggestion.




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