Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

cell-cell contact in transwell systems

cell contact transwell

  • Please log in to reply
1 reply to this topic

#1 drdesmondyap

drdesmondyap

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 19 January 2014 - 09:36 AM

I would like to conduct transwell experiments with two cell types and wants to investigate the effect of cell-cell interaction between these 2 cell types.

 

Do anyone have protocol of doing co-culture of these cells in the transwell system (ie. cell type 1 on the upper surface of the insert; while cell type 2 on the lower surface of the insert).

 

Thank you very much.



#2 MarieB

MarieB

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 20 January 2014 - 12:10 PM

Check out the "vascular cell coculture" page on this link:

 

https://www.cvrc.vir...on/methods.html

 

This is a method for coculture of endothelial cells and vascular smooth muscle cells on both sides of a transwell in order to replicate myoendothelial junctions (ie: direct contact between the two cell types) within the pores of the transwell.  I don't know if this protocol could apply to your cell types, but it might give you an idea of where to start.

 

Good luck!







Also tagged with one or more of these keywords: cell contact, transwell

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.