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Testing for DNA degradation prior to Southern Blot Experiment

DNA purification southern blot degradation protease activty

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#1 hotstuffdb22

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Posted 18 January 2014 - 05:04 AM

Hello!

Thanks to everyone who has helped me produce stable DNA from assorted mouse tissues to be eventually be used for a southern blot experiment.  I've attached a gel picture that shows 14 different undgiested genomic DNA samples that were isolated from mouse tissue.  In my opinion, all the DNA samples are fine to continue with Southern, except #4 and #12.  Each shows a fairly strong parent band, with minimal degradation underneath.  Is this conclusion correct or do you think the faint smears are a concern?

 

Thanks!

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#2 bob1

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Posted 18 January 2014 - 01:14 PM

They should be fine, even 4 and 12, though you might struggle to get enough DNA on the blots for those two (depending on how much you loaded here, and how much you have total).



#3 hotstuffdb22

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Posted 19 January 2014 - 06:18 AM

Bob1,

Thanks for your response.  I will try the experiment Mon or Tues and will hopefully update this thread with a positive result by the end of the week!







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