2 replies to this topic

[hotstuffdb22]

Hello!

Thanks to everyone who has helped me produce stable DNA from assorted mouse tissues to be eventually be used for a southern blot experiment.  I've attached a gel picture that shows 14 different undgiested genomic DNA samples that were isolated from mouse tissue.  In my opinion, all the DNA samples are fine to continue with Southern, except #4 and #12.  Each shows a fairly strong parent band, with minimal degradation underneath.  Is this conclusion correct or do you think the faint smears are a concern?

 

Thanks!

Attached Files

•  DNA Degradation Gel.pdf   100.49KB   674 downloads

[bob1]

They should be fine, even 4 and 12, though you might struggle to get enough DNA on the blots for those two (depending on how much you loaded here, and how much you have total).

[hotstuffdb22]

Bob1,

Thanks for your response.  I will try the experiment Mon or Tues and will hopefully update this thread with a positive result by the end of the week!