insert in the promoter region
Posted 18 May 2004 - 11:19 AM
I am trying to analyze the localization of one plant gene by putting a HA tag-fusion protein under the control of the native promoter. However, during my cloning, I have to add several bp (a restriction enzyme recognization site) between -1 and the ATG star codon. Do you think it will affect the normal regulation of the promoter and make the following localization inaccurate?
Posted 19 May 2004 - 05:26 PM
Posted 22 May 2004 - 06:31 PM
An Nco1 site is sometimes usefull: ccATGG