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Designing primers in UTRs

primers primer design

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#1 endlessdreamer

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Posted 17 January 2014 - 09:17 AM

Hi all,

 

I'm trying to figure out why some people design primers in untranslated regions as I want to use some published ones but they are designed for those regions. If I use them to amplify cDNA, will any genomic dna be amplified too? Could I face any problems with amplification? I read that those regions are not so stable...

 

Thanks!



#2 Inbox

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Posted 17 January 2014 - 09:28 AM

Any primer for cDNA will give amplification with genomic DNA. treat RNA with DNAase to avoid that.







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