Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Transfection mature adipocytes

Cell culturelentiviral Adipocytes Transfection 3T3

  • Please log in to reply
No replies to this topic

#1 Sergio PD

Sergio PD

    member

  • Active Members
  • Pip
  • 5 posts
1
Neutral

Posted 16 January 2014 - 12:30 PM

Hi everyone!!
 
Someone know how can I insert lentiviral particles in mature adipocytes? i follow this protocol http://www.ncbi.nlm....pubmed/14759805 but not work.
 
I have transduced  fibroblast, but i am lost with quiescent cell or mature adipocytes.
 
 why my protocol  work in growing cells but no in quiescent and mature adipocytes?
 
Thanks.

 







Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.