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Transfection mature adipocytes

Cell culturelentiviral Adipocytes Transfection 3T3

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#1 Sergio PD

Sergio PD

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Posted 16 January 2014 - 12:30 PM

Hi everyone!!
 
Someone know how can I insert lentiviral particles in mature adipocytes? i follow this protocol http://www.ncbi.nlm....pubmed/14759805 but not work.
 
I have transduced  fibroblast, but i am lost with quiescent cell or mature adipocytes.
 
 why my protocol  work in growing cells but no in quiescent and mature adipocytes?
 
Thanks.

 







Also tagged with one or more of these keywords: Cell culturelentiviral, Adipocytes, Transfection, 3T3

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