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Transfection mature adipocytes

Cell culturelentiviral Adipocytes Transfection 3T3

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#1 Sergio PD

Sergio PD


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Posted 16 January 2014 - 12:30 PM

Hi everyone!!
Someone know how can I insert lentiviral particles in mature adipocytes? i follow this protocol http://www.ncbi.nlm....pubmed/14759805 but not work.
I have transduced  fibroblast, but i am lost with quiescent cell or mature adipocytes.
 why my protocol  work in growing cells but no in quiescent and mature adipocytes?


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