Dear bioforum friends
I want to know the effect of a known SNP on binding affinity of my protein to another protein. The SNP falls exactly in the binding domain and I think it reduces the binding compared to the wild type protein. CO-IP is not really showing a big difference, I need to have a more sensitive technique.
BTW, I have cell sample from patients with the SNP and healthy control, I have extracted the protein Cell lysate for my test.
Do you know a better way than CO-IP?
Thank you guys