I have just obtained a stock of E. Coli, which I am using to test novel compounds for antibacterial activity. I am not too familiar with microbiology techniques, so there are some questions I'd like to clear up.
I plan to first streak the bacterial stock on a normal agar plate, and let the plate incubate at 37 degrees overnight. Then I will pick a colony to grow in an overnight liquid culture of Mueller Hinton broth, monitor the OD values and use the liquid solution to perform the bacterial assay.
Several questions I have:
1) Should I supplement the agar plate with any antibiotics, as to prevent the growth of other bacteria other than E. Coli? If so, what antibiotic should I use?
2) How should I maintain the agar plates so that I won't need to re-order anymore strains in the future?
Edited by pinkrainbow, 16 January 2014 - 11:57 AM.