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What method is the best to measure Telomeres?

Telomere citometry qPCR Telomeres Citometry

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3 replies to this topic

#1 Sergio PD

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Posted 16 January 2014 - 10:24 AM

Hi everyone !!

 
I just find a correlation about my protein and telomeres length =)
 
So my next step is increase  the number of sample. I have about 200 sample from patient.
 
I have read about qPCR and Flow Fish. 
 
somebody know what is more cheap? 
I understand for qPCR i need primers and no more (Syber and termocycler of course)
 
and more easy-quick? 
 
somebody use it? 
 
Can i use Flow Fish in quiescent cell? Can i use Flow Fish to tissue? 
 
if I wanna compare cell culture too. What is the best method? 
 
I have experience in qPCR and i understand very well the protocol by Cawthon but i am  beginner in FACS.
 
Thanks so much.


#2 Sergio PD

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Posted 16 January 2014 - 10:27 AM

Hi everyone !!
 
I just find a correlation about my protein and telomeres length. 
 
So my next step is increase  the number of sample. I have about 200 sample from patient.
 
I have read about qPCR and Flow Fish. 
 
somebody know what is more cheap? 
I understand for qPCR i need primers and no more (Syber and termocycler of course)
 
and more easy-quick? 
 
somebody use it? 
 
Can i use Flow Fish in quiescent cell? Can i use Flow Fish to tissue? 
 
if I wanna compare cell culture too. What is the best method? 
 
I have experience in qPCR and i understand very well the protocol by Cawthon but i am  beginner in FACS.
 
Thanks so much.


#3 bob1

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Posted 16 January 2014 - 11:48 AM

I don't know if this is outdated technology or not, but a colleague of mine used to use TeloTAGGG kits from Roche.



#4 Sergio PD

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Posted 16 January 2014 - 11:59 AM

Thank u so much for answer.

 

these kit provide 50 reaction for 770 €

 

i have 200 patients...mmmm to expensive =)







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