After working on AD-HEK293 and HEK293-T cells I switched to HEK293-E. Since these are no longer commerically available I received a sample from another lab. The only problem is my transfection yields haven't increased, although I expected improvement due to use of a new vector with an Eppstein Barr origin of replication. I have suspisions that they might not be true HEK-E cells... but how do I check?
Clearly STR profilling is the standard method, which is offered at the labs we get our sequencing performed, but will it discriminate between variants of HEK293? It appears that the profile of HEK293 and HEK293-T differ slightly but I can't find a profile for HEK-E. Does anyone know why they are no longer available for purchase? Were they originally ATCC, then Invitrogen (Life tech)? I take it they have the profile on record.
Edited by Osibisa, 14 January 2014 - 07:31 PM.