Hi, everyone, I got confused about strategy for the study of protein-protein interaction.
What I want to do is to make two fusion GPCRs with YFP and CFP, respectively, and heterdimered with each other (if they can), and next, i want use those cells transfected both plasmids to screen the compounds might have effects on the heterodimeration by FRET. I thought the plasmids should contain the full-length CDS sequence, but my boss told me that it needs only partrial CDS, like transmembrane domain, or the interaction domains coded by part of the CDS. If my boss was right, the fusion proteins lack some domain, can it be suitable for screening? Dose it make sense?
please anyone could help me