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Problem in Reviving U87MG


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#1 Annusha

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Posted 13 January 2014 - 10:13 PM

Hello everyone,

I am working with different cell lines. My U87MG stocks are not reviving following a standard protocol. Please suggest me solution for this problem.



#2 h9494036

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Posted 30 April 2014 - 11:59 AM

Someone probably froze the cells the wrong way.  Why don't you buy another vial.  Try this:  http://www.addexbio....ctdetail?pid=71



#3 Tabaluga

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Posted 30 April 2014 - 01:25 PM

@Annusha: Probably this is not an issue any more after 3.5 months but just in case you or anybody else looks it up:
1. Details of the protocol ?
2. Did you take care to thaw the cells quickly ? If it took to long this can cause problems.

Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.

 





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