I am going to subclone an ORF from a vector that has a Kozak sequence. The destination vector is an inducible lentivirus vector. Should I subclone the ORF with the Kozak sequence or just include the start codon? Kozak sequence will enhance translation but will it cause problems in a inducible lentivirus vector? Or will the lack of the kozak sequence have a minimal impact on translation efficiency? Goal is to produce lentivirus from this vector and transduce mammalian cells.