Dear All, I have a question about the Cre-Lox recombination technology. When a piece of DNA is flanked by a pair of inverted LoxP sites, the DNA will be inverted in the presence of Cre recombinase. I was just wondering, since the LoxP sites are retained after recombination (unlike when they are in the same orientation; one of the two is excised with the DNA), wouldn't this result in reversion of the DNA back to its original orientation if Cre recombinase is still present?
Also, I know that tissue culture-grade Cre recombinase is available commercially, but does anyone have any experience using it in suspension cultures?
Thanks in advance!