Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

seeking for FISH handbook etc

fluorescent in situ hybr

  • Please log in to reply
6 replies to this topic

#1 LAHAV30

LAHAV30

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 13 January 2014 - 05:55 AM

Can someone redirect me please for a good free resource for a FISH handbook or even a website where i can find

 

the basic of FISH and the principle behind it. for example - when you pre-wash slides with 2Xssc, what does it do to a cells, tissue etc?

 

Many thanks,

 

Lahav.

 

 



#2 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,757 posts
130
Excellent

Posted 14 January 2014 - 04:31 AM

you can start here: wikipedia article on FISH

 

then go to this website for explanations and protocols: genedetect


talent does what it can
genius does what it must
i do what i get paid to do

#3 Ameya P

Ameya P

    Rervm Cognoscere Cavsas

  • Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 330 posts
25
Excellent

Posted 18 January 2014 - 02:04 AM

You might also want to visit Tavi's  FISH Guide.  I have found it quite useful most of the times. 


NEW!!!!  The Beauty in Symmetry on CoffeeTableScience!!!!

Image copyright: Adrian Koh SF.
Replication of this art is strictly prohibited without express permission of the artist


#4 LAHAV30

LAHAV30

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 18 January 2014 - 11:55 PM

thx for all answers, but still i'm looking for some even more detailed info about the FISH technique. Tavi's  FISH Guide is great but not detailed enough for me.

 

any how, can someone answer me if 70% denatured ethanol damage FISH on cell suspension?



#5 Ameya P

Ameya P

    Rervm Cognoscere Cavsas

  • Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 330 posts
25
Excellent

Posted 25 January 2014 - 03:45 AM

Could you please reframe that question. I did not completely understand, what you are asking. 


NEW!!!!  The Beauty in Symmetry on CoffeeTableScience!!!!

Image copyright: Adrian Koh SF.
Replication of this art is strictly prohibited without express permission of the artist


#6 LAHAV30

LAHAV30

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 25 January 2014 - 09:35 PM

Ameya P, you didn't understand my last question or the first one?

 

I'm doing FISH on cell suspension from sputum and would like to know if ethanol denatured can damage Hybridisation (weak signals) or not?



#7 Ameya P

Ameya P

    Rervm Cognoscere Cavsas

  • Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 330 posts
25
Excellent

Posted 01 February 2014 - 04:43 AM

LAHAV30, 

 

when you are washing your sample after application of the probe and the hybridisation duration, ethanol is merely helping you dehydrate the slide. It does not have any effect on the signal strength that you are getting. For weaker signals, your should look at the sample preparation, denaturation and hybridisation steps for slip ups. 


NEW!!!!  The Beauty in Symmetry on CoffeeTableScience!!!!

Image copyright: Adrian Koh SF.
Replication of this art is strictly prohibited without express permission of the artist





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.