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Calculating transfection index?

transfection viability

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#1 cutyla

cutyla

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Posted 13 January 2014 - 04:37 AM

Hello, So I know the transfection index works in relation to calculating the ratio of transfection efficiency and cell viability, can anyone give me more details?

 

For example if I transfected cells with GLuc and measured the efficiency and then measured viability by Alamar blue?

 

I think the efficiency needs to be normalized to the cell number but I can't find any more details on the standard way to do this.

Thanks for any help!






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