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prevent cel detachment

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#1 Dashneh



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Posted 10 January 2014 - 04:01 AM



I am facing a problem with MCF7 cell lines attachement to slides coated with poly-l-lysine and superfrost slide. I start to smear the solution on glass slide and let it dry afterwds the slide is mounted with coverslip and vectashield mounting medium hard-set and not hardset. but when i try to unmount the coverslip from the slide the cells start to detach from the surface and I end up with no cells on my slide. Any suggestions on why and how can i prevent the cells from detache and remove from the slide?


kind regards

#2 bob1


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Posted 12 January 2014 - 01:48 PM

Why do you mount the coverslips and then remove them?  It is not recommended for cells grown on slides, as they will usually detach or be damaged.

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