Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Help help

prevent cel detachment

  • Please log in to reply
1 reply to this topic

#1 Dashneh

Dashneh

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 10 January 2014 - 04:01 AM

Hello...

 

I am facing a problem with MCF7 cell lines attachement to slides coated with poly-l-lysine and superfrost slide. I start to smear the solution on glass slide and let it dry afterwds the slide is mounted with coverslip and vectashield mounting medium hard-set and not hardset. but when i try to unmount the coverslip from the slide the cells start to detach from the surface and I end up with no cells on my slide. Any suggestions on why and how can i prevent the cells from detache and remove from the slide?

 

kind regards



#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,725 posts
399
Excellent

Posted 12 January 2014 - 01:48 PM

Why do you mount the coverslips and then remove them?  It is not recommended for cells grown on slides, as they will usually detach or be damaged.






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.