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bacterial transformation kit life-technologies

bacterial transformation

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#1 trangtrang



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Posted 10 January 2014 - 02:28 AM

Hi you guys, 


I did my transformation yesterday. I supposed that pUC19 supplied in the kit is for the positive control. 

Strikingly, I had few (around 50 colonies) in this positive control (pUC19) but a several hundreds with my plasmid (after gateway LR reaction). 


Do you have any explanation for my case? 


Thanks in advance.



#2 dimensionsbio



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Posted 11 January 2014 - 08:35 AM

Hi Trang.  Unless your plasmid was also pUC19 and you used the exact same amount, then you are not really looking at a comparable control.  Colony counts can be interesting to do, but they rarely provide any useful information.  The results from a screen (either digest or PCR) of colonies on your experimental plate are more important.

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